Nascent poly(fl-hydroxybutyrate) (PHB) granules are found in most bacterial strains and can be isolated as a milky suspension. Their structure was examined by transmission and scanning electron microscopy and has been shown to be made of two distinct components: a solid shell composed of overlapping lamellar crystals and a soft non-crystalline core. It was possible to obtain a ductile deformation of the core in liquid nitrogen even though the glass transition of isotactic PHB approaches 0°C. Finally, glycerol triacetate, a minor component of the granule, was found to be a good solvent for PHB from which metastable solutions can be obtained. Self-seeding of these solutions led to the formation of lamellar crystals comparable to the structure of the shells
Посилання на статтю:
Structural study of isolated poly (fl-hydroxybutyrate) granules / C. Lauzier and R. H. Marchessault // Polymer. – 1992. – Vol 33. – P. 823-827.