The interference of allylic hydroperoxides in the reaction of 2,4-dinitrophenylhydrazine (DNPH) with oxidized polyisoprene was investigated. During the DNPH analysis, hydroperoxides were susceptible to acid-catalysed Hock cleavage, producing carbonyl compounds which subsequently reacted with DNPH to form additional hydrazone derivatives. Prior reduction of the hydroperoxides with triphenylphosphine (PPh3) appeared to be a satisfactory method of eliminating this problem, giving more accurate carbonyl levels.
Посилання на статтю:
The determination of carbonyls as their 2,4-dinitrophenylhydrazine (DNPH) derivatives in peroxide-containing polymers / A. R. Arnold* and R. Staples // Polymer. – 1992. – Vol 33. – P. 1739-1741.