Optically controlled ligand delivery systems would be useful for the design and development of biosensors. We synthesized a N-(2-hydroxypropyl)methacrylamide (HPMA) copolymer containing sidechains terminated in model ligand (Boc-Gly), bound via photocleavable 2-nitrobenzyl bonds. To study photocleavage at solid liquid interfaces, the HPMA copolymer was covalently attached to 3-aminopropyl triethoxy silane (APS)-coated porous silica beads, which were modified with ct,~o-diaminopoly(ethylene oxide) of molecular weight 1000 or 5000. The influence of the poly (ethylene oxide) (PEO) spacer on the rate of photolysis and on the minimization of non-specific adsorption was determined. The results show that the photolytic rates are faster for copolymer-derivatized silicas with PEO spacers compared to that without spacer and that the PEO spacer reduces the non-specific adsorption of HPMA copolymer on the silica surface.
Посилання на статтю:
Optically controlled ligand delivery: 3. Photocleavage of 2-nitrobenzyl bonds at solid-liquid interfaces / Hung-Ren Homer Yen, Joseph D. Andrade and JindPich Kopebek // Polymer. – 1992. – Vol 33. – P. 1763-1767.