Macroporous beaded polymers of varying pore sizes were synthesized coated with polyethyleneimine and derivatized with glutaraldehyde to generate aldehyde pendant groups. Penicillin G acylase (EC 220.127.116.11) was immobilized by covalent binding and the performance of immobilized enzyme was evaluated in batch mode. The expression was estimated as 43%. The shift in pH optima was marginal. The optimum temperature shifted from 40 to 57°C and Km shifted from 31 to 12.8/~mol. Immobilization enhanced the thermal stability of the enzyme.
Посилання на статтю:
Immobilization of penicillin G acylase on functionalized macroporous polymer beads / R. V. Bahulekar*, A. A. Prabhunet, H. SivaRamant and S. Ponrathnam // Polymer. – 1993. – Vol 34. – P. 163-166.