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Macroporous beaded polymers of varying pore sizes were synthesized coated with polyethyleneimine and derivatized with glutaraldehyde to generate aldehyde pendant groups. Penicillin G acylase (EC 3.5.1.11) was immobilized by covalent binding and the performance of immobilized enzyme was evaluated in batch mode. The expression was estimated as 43%. The shift in pH optima was marginal. The optimum temperature shifted from 40 to 57°C and Km shifted from 31 to 12.8/~mol. Immobilization enhanced the thermal stability of the enzyme.

Посилання на статтю:

Immobilization of penicillin G acylase on functionalized macroporous polymer beads / R. V. Bahulekar*, A. A. Prabhunet, H. SivaRamant and S. Ponrathnam // Polymer. – 1993. – Vol 34. – P. 163-166.

Immobilization of penicillin G acylase on functionalized macroporous polymer beads - Завантажити.