A recently developed in vitro procedure for preparing artificial granules of microbial polyesters has been applied to a blend of poly(3-hydroxybutyrate) (PHB) and poly(3-hydroxyoctanoate) (PHO). Density gradient analysis showed that these granules formed a single, uniform population; n.m.r, showed that the polymers retained their amorphous elastomeric state; and thermal analysis indicated that the polymers formed separate phases within these granules. These results contrast strongly with the observation that a recombinant bacterial strain simultaneously expressing PHB and PHO stores the two polymers in separate cellular granules. The implications of the in vitro findings for granule formation in vivo are discussed.
Посилання на статтю:
Phase separation within artificial granules from a blend of polyhydroxybutyrate and polyhyd roxyoctanoate: biological implications / Daniel M. Horowitz and Jeremy K. M. Sanders // Polymer. – 1994. – Vol 35. – P. 5079-5083.