Partition coefficients obtained from liquid chromatography are compared to those obtained from equilibrium sorption experiments for various alcohol-water-cellulose acetate systems. In the liquid chromatography experiments cellulose acetate powder was used as the stationary phase with water as the mobile phase and alcohols injected into the mobile phase. In the equilibrium sorption experiments, cellulose acetate power was equilibrated with an alcohol-water solution. The distribution coefficients determined using liquid chromatography and those determined using liquid phase sorption exhibit similar trends with changing solute structure. However, the chromatographic distribution coefficients are, in general, smaller than the equilibrium distribution coefficients. This discrepancy is attributed to the fact that equilibrium is not attained in the chromatographic column. The fact that the distribution coefficients are in reasonable agreement indicates that while the kinetics of diffusion prevent a true equilibrium distribution coefficient from being obtained from the chromatography column, the results of the chromatographic experiments give a qualitative representation of the affinity of the polymer phase for each solute
Посилання на статтю:
A comparative study of partition coefficients determined by sorption and by liquid chromatography in alcohol-water-cellulose acetate systems / Timothy B. Meluch and Douglas R. Lloyd // Polymer. – 1993. – Vol 34. – P. 1984-1987.